Okay, I've read through all the abstracts (I wish I could read the whole articles, but even my membership with ACS doesn't cover that) plus some other articles, too. There's a great danger in only reading the abstracts -- in some of the articles, it was not clear whether "pu-erh" meant: raw, aged, or ripe. There's a lot of other critical info missing (which I'll talk about)
Bottom line -- I don't see anything that suggests young pu-erh to be bad. Or at least, if it is, then drinking Chinese green tea is just as bad. In all things moderation.
Let me address the concerns in the most obvious article - the one that looked at tea extract effects on rats (J Agric Food Chem,
2010,
58 (2), 1350-8.
The first problem is that without looking at the whole article, we have no idea what "Green Tea Extract" and "Black Tea Extract" actually mean. Did they just use boiling water? Did they use ethanol? (another study did use 75% ethanol for extraction) Did they use "normal brewing amounts?" or more? less? All these things will affect the concentration of the "extract." It's also unclear, then, if they reduced it at all (i.e. removed some water).
The next problem is the
amount that they fed the rats. Now, this was explicitly
supposed to be a high-dose study. But look at these amounts: 2500 mg/kg/day and 5000 mg/kg/day.
What does this mean? It means a rat was fed 2500 mg (or 2.5 grams) of extract
per kilogram of its body weight every day (for 28 days in this study). For reference, I weigh 200 lbs, or 90 kg. This means I would be taking (2.5 * 90) = 225 grams, or (5.0 * 90) = 450 grams of extract every day. Now you can see why it's important to know what "extract" means. Is it 225 grams of a normal tea solution? (which is only about 7.5 oz... not a lot), or is it something else?
Finally the results -- "low toxicity." They used two assays that showed an increase in activity (they don't say how much it increased) -- as Mr. Usagi asks, does this mean liver damage? Well, the alanine amino transferase increase can be an indication of liver damage. But notice they say that the target organs were "considered" to be the liver and the kidney. In other words, it's a possibility. They do not mention an LD50 (lethal dose that kills half the population) -- in fact, it doesn't sound like any of the rats died at all (well, except maybe at the end of the study).
Of course, the real importance here is the comparison of the raw pu-erh with a fermented pu-erh, which under the same conditions, shows "no adverse effects...." That makes fermented pu "better."
We can't really resolve this one until we get a better definition of what "extract" meant. I am keenly aware of this problem, because a study just like this is why sassafras is banned from sale in the U.S. Somebody did a study with massively high concentrations on rats (equivalent of gallons per day) and the negative effects on the rats resulted in a ban.
But aside from the danger aspect, we talked about a number of other things. One of the other issues had to do with acids. The problem is that
almost all of these frickin' compounds are acids. EGCG, catechins, and all the derivatives are acids (phenolic acids). But compared to ascorbic acid, citric acid, and a number of other acids common in food and our bodies, these phenolic acids are
weak. You get to stronger acids when you start to talk about gallic acid, but according to at least two articles, gallic acids increases in pu-erh tea as it ages (which makes sense, and it would form from the oxidation of EGCG). This is nothing to worry about.
I noticed the introduction of an interesting term -- theabrownin. Only one source identified this term -- as "a group of water soluble polyphenols exhibiting brown color" (hence the name). In fact, one of these (the "-omics" 2-page paper) claims it's the major component of pu-erh. I would like to know more about this if possible. As best as I can tell, it is another word for ... tannins.
Which brings me to one of the missing pieces of
all of these articles -- tannins. Or what you might call polymeric polyphenols. Here's a description of the chemicals in layman's terms. You've got gallic acid, and you've got flavone. Call them A and B. Catechins and all their ilk are derivatives (slight changes) to B. If you attach A to B (and make a few minor modifications) and you get the well-known EGCG (and all of its variations). If two of B dimerize (join together) you get theaflavin. There are lots of combinations, rather like lego blocks (you can even throw on sugars to these things), and they can keep forming really long links -- when they get big, those are what are collectively called tannins.
The problem with these really long chains of things -- they're hard to identify. Think of it this way. You've got all the building blocks A, B, and the slight changes (call them A', A'', B', B'', etc). It's "easy" to detect those because they're small and single units.
But when you start combining them (AA, AA', AB', AB'AB), each of these things is unique, and the possible combinations become staggering -- which is why the long chains just get called "tannins."
All of these studies appear to be using HPLC (high pressure liquid chromatography). For a quick description -- imagine sending a flood of people through an obstacle course. People have different sizes, and some have to hold hands as they go through. The smaller, quicker people will make it through first, while the larger, or linked people will take longer (as they get wrapped around something and have to disentangle, etc) -- a goofy description, but good enough. The researchers will have "standards" for comparison of the simple small building blocks (like EGCG, gallic acid, catechin, etc). But they won't for the more complicated combinations. In fact, having experience in this, I almost guarantee that all those many combinations all smear out as a nasty blob on their machine. Polymer-type compounds rarely play well on chromatography systems.
Bottom line on this tannin discussion -- they may be looking at the little bits left over when there are large structures sitting there that they're missing.
Very finally, as to the question of pesticides -- I would like to see a reference as to the break-down of these pesticides. One of the notorious things about pesticides is that they're often
stubborn in degrading. We've come a long way since the days of DDT, or at least, we have in America.
Whew, well this was an interesting Saturday. I probably buried all my concerns in too many words.... but Nicolas, thanks again for all the interesting articles. I learned a lot. And most importantly, I learned not to worry.
edit: and I see while I was writing up this novel, debunix brought up the same points. Bingo!
